MRes Studentship: Targeted Genome Editing in Cannabis Sativa
Overview
- Qualification type: MSc by Research
- Subject area: Targeted Genome Editing in Cannabis Sativa
- Location/Campus: College Lane Campus, Hatfield
- Closing application date: 30 September 2024
- Start date: 1 November 2024 or as soon as possible thereafter.
- Duration: one year, full time
Description
This is an exciting opportunity to undertake a fully funded one-year Master’s by Research project, in collaboration between the University of Hertfordshire and Precision Plants. This project aims to develop advanced genome editing techniques for Cannabis sativa using CRISPR-Cas9 and Agrobacterium infection. The focus is on creating THC-free cannabis cultivars by optimising genetic engineering strategies to modify cannabinoid biosynthesis, with the ultimate goal of producing cannabis varieties suitable for therapeutic use.
Key responsibilities and duties
The goal of this project is to develop genome-edited (GE) cannabis cultivars with optimised ratios of targeted therapeutic cannabinoids (such as CBD) and the absence of THC, for medical and wellness applications. The Student Associate will implement explant micropropagation techniques (in vitro tissue culture), design gene editing strategies using CRISPR/Cas9, and enhance Agrobacterium-mediated infection techniques for commercially available cannabis cultivars. Additionally, computational approaches will be used to develop and optimise genetic engineering strategies applicable to multiple cannabis cultivars.
Eligibility
Applicants must be UK nationals or have permanent UK residence status. Overseas candidates may be considered, however must cover the difference between domestic and international tuition fees and obtain a student visa.
The successful candidate will need to demonstrate the following:
Essential
- A first- or second-class UK honours degree or equivalent in a relevant discipline within the sciences (i.e. Biosciences).
- Knowledge and practical experience of working within a scientific laboratory (weighing, pipetting, filtration, preparation of solutions).
- Ability to prioritise work independently as well as being part of a wider research group delivering research objectives.
- Applicant must demonstrate their ability to project manage and undertake research through the preparation, setting up, conducting, and recording of experiment outcomes.
- Excellent oral and written English communication skills, including the ability to communicate with clarity on complex information.
- Good interpersonal skills.
- An ability to manage competing tasks whilst maintaining motivation and engagement.
- Must be able to demonstrate sufficient breadth and depth of knowledge within the particular research field.
Desirable
- Interest in the agricultural industry and working in a commercial environment.
- Knowledge of statistical approaches to experimental design and analysis.
- Experience working on plant molecular science and on in vitro culture plant systems.
- Good molecular biology, technical and bioinformatic skills, focused on cloning systems and sequence analysis.
The successful applicant will need to have the willingness and ability to undertake an industrial placement and when appropriate, and to have a flexible approach to travel in order to attend training activities when required.
Supervisor Information
- Principal Supervisor: Dr Cristina Barrero Sicilia, University of Hertfordshire
- Second Supervisor: Dr Jacqueline Stair, University of Hertfordshire
- Company Supervisor: Charles Clowes, Precision Plants
How to apply
Applicants must provide the following:
- A completed application form.
- Two academic references to be provided by referee directly to the Doctoral College.
- Copies of qualification certificates and transcripts.
- Certification of English language competence (minimum IELTS 6.5 or equivalent) for candidates for whom English is not their first language.
Please send completed applications to the Doctoral College, using the following in the email subject line: Targeted Genome Editing in Cannabis sativa via CRISPR-Cas9 using Agrobacterium Infection.
The closing date for applications is 30th September 2024. Shortlisted applicants will be invited to an interview W/C 7th October 2024
Informal Enquiries
Informal enquiries should be addressed to Dr Cristina Barrero Sicilia, but please note that applications sent to this address will not be considered.
Funding
The award includes cover for UK home tuition fees and subject to eligibility, a stipend at standard UKRI rates. For 2024-25 this is set at £19,237.